mapk signaling pathway  (Selleck Chemicals)

Journal: Immunobiology

Article Title: Giardia duodenalis triggered neutrophil extracellular traps in goats.

doi: 10.1016/j.imbio.2025.152894

Figure Lengend Snippet: Fig. 6. NETs release and ROS production was associated with NADPH oxidase, ERK1/2 and p38 MAPK signaling pathway (A) NETs quantitation. (B) ROS level. Goat neutrophils were pretreated with NADPH oxidase inhibitor DPI (10 μM), ERK1/2 inhibitor U0126 (50 μM), p38 MAPK signaling pathway inhibitor SB202190 (10 μM) respectively for 30 min, and stimulated with Giardia (ratio 1:1) for 2 h. Zymosan (1 mg/mL) was used as a positive control. Data were expressed as mean ± SD (n = 5). P < 0.05 was considered significant (*P < 0.05, ***P < 0.001).

Article Snippet: In inhibition tests, prior to stimulation with G. duodenalis (at a ratio of 1:1), neutrophils were pretreated with 10 μM of the NADPH oxidase inhibitor Diphenyleneiodonium chloride (DPI, Sigma-Aldrich, USA), 50 μM of ERK1/2 inhibitor U0126 (Sigma-Aldrich, USA), 10 μM of the p38 MAPK signaling pathway inhibitor SB202190 (Sigma-Aldrich, USA), 100 μM of TLR2 inhibitor C29 (MedChemExpress, USA) and 10 μM of TLR4 inhibitor TLR4-IN-C34 (C34, MedChemExpress, USA) respectively for 30 min 30 min. Zymosan was used as the positive control at a concentration of 1 mg/mL.

Techniques: Quantitation Assay, Positive Control

Journal: Advanced Science

Article Title: Enhancing Tendon Regeneration: Investigating the Impact of Topography on the Secretome of Adipose‐Derived Stem Cells

doi: 10.1002/advs.202417447

Figure Lengend Snippet: ACM improves cell proliferation and tendon differentiation of TSPCs by activating MAPK and Integrin pathways. A) Western blot of p‐MEK and MEK in TSPCs after treatment of RCM, RCM+U0126, ACM, or ACM+U0126 for 18 h. B) Western blot of p‐ERK, ERK in TSPCs after treatment of RCM, RCM+U0126, ACM, or ACM+U0126 treatment for 18 h. C) Gene expression of PCNA in different groups at day 3. D) Proliferation of TSPCs measured by CCK‐8 at 1, 3, and 5 days, n = 5 technically independent samples for each group, ** p < 0.01 **** p < 0.0001. E) Gene expression of SCX, TNMD, and MKX in different groups at day 3. Levels at the RCM group were set as 1. Data were shown as Mean ± SD, n = 3 technically independent samples for each group, * p < 0.05, ** p < 0.01. F) Gene expression of PCNA in different groups at day 3, *** p < 0.001. G) Proliferation of TSPCs measured by CCK‐8 at 1, 3, and 5 days, n = 5 technically independent samples for each group, **** p < 0.0001. H) Gene expression of SCX, TNMD, and MKX in different groups at day 3. Levels at the RCM group were set as 1. Data were shown as Mean ± SD, n = 3 technically independent samples for each group, * p < 0.05.

Article Snippet: When TSPCs reached to 90% confluence, the cells were treated with CMs or CMs combined with 20 μ m U0126 (Selleck, S1102) to inhibit MAPK signaling pathway, or CMs combined with 40 μ m RGD peptides (Selleck, S8008) to inhibit integrin signaling pathway.

Techniques: Western Blot, Gene Expression, CCK-8 Assay

Journal: Journal of Advanced Research

Article Title: Extracellular vesicles derived from Lactobacillus johnsonii promote gut barrier homeostasis by enhancing M2 macrophage polarization

doi: 10.1016/j.jare.2024.03.011

Figure Lengend Snippet: Effect of LjEVs on macrophage MAPK signaling pathway and macrophage polarization in vitro .(A) Gene expression of Arg1, IL-4, IL-10, and CD206 in J774A.1 cell. (B) mRNA expression of IL-1β, TNF-α, ZO-1, and Occludin in MODE-K cells in the co-culture system. (C) KEGG pathway enriched between the CON and ETEC K88 groups or between the ETEC K88 and ETEC K88 + LjEVs groups. (D) Protein expression and analysis of the MAPK signaling pathway in J774A.1 cell. (E) Arg1, IL-4, IL-10, and CD206 gene expression in J774A.1 cell. (F) IL-1β, TNF-α, ZO-1, and Occludin mRNA expression in MODE-K cells in the co-culture system. The data were presented as means ± SEM. * P < 0.05.

Article Snippet: For the effect of MAPK signaling pathway inhibitor on macrophage phenotype experiment, J774A.1 cells were incubated with SB203580 (Selleck, S1076; 10 μM) or SCH772984 (Selleck, S7101; 2 μM) or SP600125 (Selleck, S1460;10 μM) for 3 h, then incubated with ETEC K88 (10 8 CFU/mL) for 4 h. The cells and cell supernatant were harvested.

Techniques: In Vitro, Gene Expression, Expressing, Co-Culture Assay

Journal: Journal of Advanced Research

Article Title: Extracellular vesicles derived from Lactobacillus johnsonii promote gut barrier homeostasis by enhancing M2 macrophage polarization

doi: 10.1016/j.jare.2024.03.011

Figure Lengend Snippet: Effect of MAPK signaling pathway blocked macrophage on NLPR3 signaling pathway and intestinal barrier in intestinal epithelial cells in vitro . (A) Concentrations of IL-1β, TNF-α, IL-10, and TGF-β in J774A.1 cell supernatants. (B) Gene expression of Arg1, IL-4, IL-10, and CD206 in J774A.1 cell. (C) KEGG enrichment pathway in MODE-K cell in the co-culture system. (D) Transcriptome-based differential gene expression associated with inflammatory responses. (E) Protein expression and analysis of the NLRP3 signaling pathway and tight junctions in MODE-K cells in the co-culture system. The data were presented as means ± SEM. * P < 0.05.

Article Snippet: For the effect of MAPK signaling pathway inhibitor on macrophage phenotype experiment, J774A.1 cells were incubated with SB203580 (Selleck, S1076; 10 μM) or SCH772984 (Selleck, S7101; 2 μM) or SP600125 (Selleck, S1460;10 μM) for 3 h, then incubated with ETEC K88 (10 8 CFU/mL) for 4 h. The cells and cell supernatant were harvested.

Techniques: In Vitro, Gene Expression, Co-Culture Assay, Expressing